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Working with a Spectrophotometer
Description
Measuring the amount of light emitted or absorbed by a substance allows for the determination of its concentration and chemical identity through the use of a spectrophotometer. This video demonstrates the correct procedure for operating the device, from performing a baseline calibration using a "blank" to measuring samples in specialized cells (cuvettes) to ensure accurate results.
Original title
עבודה עם ספקטרופוטומטר
מדידת כמות האור שפולט או בולע חומר מאפשרת לקבוע את ריכוזו ואת זהותו הכימית באמצעות מכשיר הספקטרופוטומטר. הסרטון מדגים את אופן העבודה הנכון עם המכשיר, החל מאיפוסו בעזרת 'בלנק' ועד למדידת דגימות בתאים ייעודיים (קוובטות) לקבלת תוצאות מדויקות.
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