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FRET and Fluorescence Resonance Energy Transfer 2010
Description
The FRET process enables the measurement of distances between molecules on a nanometric scale through radiationless energy transfer between two fluorescent molecules. The method relies on high sensitivity to the distance between a "donor" and an "acceptor," allowing researchers to investigate dynamic biological processes and structural changes in proteins within living cells.
Original title
FRET - העברת אנרגיה בתהודה פלואורסצנטית (03-05-2010)
תהליך ה-FRET מאפשר למדוד מרחקים בין מולקולות בסקלה ננומטרית באמצעות מעבר אנרגיה ללא פליטת קרינה בין שתי מולקולות פלואורסצנטיות. השיטה מתבססת על רגישות גבוהה למרחק בין "תורם" ל"מקבל", דבר המאפשר לחקור תהליכים ביולוגיים דינמיים ושינויי מבנה של חלבונים בתוך תאים חיים.
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