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Molecular Cloning
Description
The molecular cloning process enables the creation of multiple copies of specific DNA segments, involving the isolation of a gene and its insertion into a plasmid. By utilizing restriction enzymes and ligase, scientists can engineer genetic sequences and introduce them into bacterial cells to study their function or to produce proteins in large quantities.
Original title
שיבוט מולקולרי (2011-05-03)
תהליך השיבוט המולקולרי מאפשר ליצור עותקים רבים של מקטעי DNA ספציפיים וכולל בידוד של גן והחדרתו לתוך פלסמיד. באמצעות אנזימי הגבלה וליגאז, מדענים יכולים להנדס רצפים גנטיים ולהחדירם לתאי חיידקים כדי לחקור את תפקודם או לייצר חלבונים בכמויות גדולות.
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